Biochemistry Lab

Biochemistry lab I. Introduction In this experiment, lipids from ground nutmeg ar extracted using a combination of firmness of purposes and identify the lipids by chromatography. The use of goods and services of using solvent combinations is to elute the lipids establish on their mutual opposition to binding of the silica jelly. The chromatography is performed on a silica change plate and the use of iodin to visualize the lipids. By scheming the Rf values for for each one compound and comparing them to the know lipids, we are able to rush laid the lipids within the grounded nutmeg. II. Procedures Add 5 g crushed nutmeg and 50 mL hexane-isopropanol into a flask and warm for 15 minutes. Remove the extra solvent on a locomote bath under a hood while flushing the flask with N2 gas, leaving the crude extract. consult extract. Prepare silica gel column. Add 6 g of silica gel in 20 mL of hexane to read a slurry. Blo ck column with small piece of deoxyephedrine wool, add 5 mL of hexane and then add the silica slurry up t...If you want to get a full essay, show it on our website: OrderCustomPaper.com

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